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Growth Factor

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Growth Factor last won the day on November 19 2018

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  1. lmao @ the last thought. An allosteric modulator is just a term for things that interact with an enzyme or receptor - but not via the ligand-docking site - and cause a conformational change to the enzyme leading to modulation of the enzyme's function. Apologies if you meant you don't remember the exact compounds instead of what an allosteric modulator is.
  2. Thomas D. Prevot, Guanguan Li, Aleksandra Vidojevic, Keith A. Misquitta, Corey Fee, Anja Santrac, Daniel E. Knutson, Michael Rajesh Stephen, Revathi Kodali, Nicolas M. Zahn, Leggy A. Arnold, Petra Scholze, Janet L. Fisher, Bojan D. Marković, Mounira Banasr, James M. Cook, Miroslav Savic, Etienne Sibille. Novel Benzodiazepine-Like Ligands with Various Anxiolytic, Antidepressant, or Pro-Cognitive Profiles Molecular Neuropsychiatry, 2019; 1 DOI: 10.1159/000496086 Abstract Altered gamma-aminobutyric acid (GABA) function is consistently reported in psychiatric disorders, normal aging, and neurodegenerative disorders and reduced function of GABA interneurons is associated with both mood and cognitive symptoms. Benzodiazepines (BZ) have broad anxiolytic, but also sedative, anticonvulsant and amnesic effects, due to nonspecific GABA-A receptor (GABAA-R) targeting. Varying the profile of activity of BZs at GABAA-Rs is predicted to uncover additional therapeutic potential. We synthesized four novel imidazobenzodiazepine (IBZD) amide ligands and tested them for positive allosteric modulation at multiple α-GABAA-R (α-positive allosteric modulators), pharmacokinetic properties, as well as anxiolytic and antidepressant activities in adult mice. Efficacy at reversing stress-induced or age-related working memory deficits was assessed using a spontaneous alternation task. Diazepam (DZP) was used as a control. Three ligands (GL-II-73, GL-II-74, and GL-II-75) demonstrated adequate brain penetration and showed predictive anxiolytic and antidepressant efficacies. GL-II-73 and GL-II-75 significantly reversed stress-induced and age-related working memory deficits. In contrast, DZP displayed anxiolytic but no antidepressant effects or effects on working memory. We demonstrate distinct profiles of anxiolytic, antidepressant, and/or pro-cognitive activities of newly designed IBZD amide ligands, suggesting novel therapeutic potential for IBZD derivatives in depression and aging. https://www.karger.com/Article/Abstract/496086 A press release regarding this research had the following to say I'm unfamiliar with the field of cognitive decline and medications that combat or even reverse such conditions, but the abstract makes this appear as something interesting. I can't access the FT right now. If anybody else has resources about drugs in this field I'd be interested to gloss over those. Thanks.
  3. Growth Factor

    Best exercise for general neck hypertrophy?

    I could have sworn @JudoJosh had a whole ebook dedicated to the neck. @Emperor G_D you don't have something like this on the old drive?
  4. Yeah, so in my very limited experience physicians really don't like to give elective blood tests outside of what's done for a physical. I wont dare pass judgment on that front because it's possible thousands of patients ask for all the same things you or I are interested in, but it's only relevant to 1% of the patients. I don't know. Anyways, knowing this all, I say just go ahead and see what the numbers are first. It's very expensive to look at other parameters if you don't have a documented underlying condition where these tests are indicated. If he's already thrown on TT, FT, and E2, then maybe the only other thing I would ask for is SHBG. Ordinarily, physicians will just order a basic hormone panel that includes TT, LH, and FSH. If nothing is abnormal and you don't* feel fine, there may be follow up tests done to tease out what's going on further. *edit: a word
  5. SHBG, FSH, and LH should always be looked at if you're already interested enough in monitoring other hormones like FT. It would help to know why you're getting the bloodwork. If you're healthy and just want a baseline, then I'd get those done + LH. Cortisol isn't done by blood/isn't reliable/doesn't mean anything, so I wouldn't worry about that at all unless you're trying to diagnose or monitor an underlying condition. Cortisol changes throughout the day based on tens or hundreds of things, so the test people give for cortisol is generally a cortisol challenge where you collect saliva (my bad, sputum has mucus) across various timepoints throughout a day. But this is very annoying and unnecessary for a healthy individual. Lipids and blood chemistry is always good to get and usually covered by insurance, so I'd opt for those if you have insurance.
  6. Growth Factor

    Proper CBD dosage?

    To be fair, I have only read the abstract for the following since I'm at home, but I just came across this... Low dose CBD synergizes with THC, particularly in drug-naive individuals, while high dose CBD counteracts the intoxicating effects of THC: https://link.springer.com/article/10.1007%2Fs00406-019-00978-2 A randomised controlled trial of vaporised Δ9-tetrahydrocannabinol and cannabidiol alone and in combination in frequent and infrequent cannabis users: acute intoxication effects Nadia SolowijEmail authorSamantha BroydLisa-marie GreenwoodHendrika van HellDave MartelozzoKuna RuebJuanita ToddZheng LiuPeter GalettisJennifer MartinRobin MurrayAlison JonesPatricia T. MichieRodney Croft First Online: 19 January 2019 Abstract Access to cannabis and cannabinoid products is increasing worldwide for recreational and medicinal use. Two primary compounds within cannabis plant matter, Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD), are both psychoactive, but only THC is considered intoxicating. There is significant interest in potential therapeutic properties of these cannabinoids and of CBD in particular. Some research has suggested that CBD may ameliorate adverse effects of THC, but this may be dose dependent as other evidence suggests possible potentiating effects of THC by low doses of CBD. We conducted a randomised placebo controlled trial to examine the acute effects of these compounds alone and in combination when administered by vaporisation to frequent and infrequent cannabis users. Participants (n = 36; 31 male) completed 5 drug conditions spaced one week apart, with the following planned contrasts: placebo vs CBD alone (400 mg); THC alone (8 mg) vs THC combined with low (4 mg) or high (400 mg) doses of CBD. Objective (blind observer ratings) and subjective (self-rated) measures of intoxication were the primary outcomes, with additional indices of intoxication examined. CBD showed some intoxicating properties relative to placebo. Low doses of CBD when combined with THC enhanced, while high doses of CBD reduced the intoxicating effects of THC. The enhancement of intoxication by low-dose CBD was particularly prominent in infrequent cannabis users and was consistent across objective and subjective measures. Most effects were significant at p < .0001. These findings are important to consider in terms of recommended proportions of THC and CBD in cannabis plant matter whether used medicinally or recreationally and have implications for novice or less experienced cannabis users.
  7. Growth Factor

    Proper CBD dosage?

    Highly relevant blog/article about these CBD products on the market: https://medium.com/@julie.meade/pharmtox-lab-rats-volume-4-imitation-marijuana-d0d05c67ed01 While the article focuses on designer cannabinoids, there's a section about CBD products. The CBD market seems to have all the same pitfalls as the supplement market does/did years ago, and many products not only don't appear to have a therapeutic dose of CBD but are tainted with other compounds. As always, shop responsibly.
  8. Growth Factor

    Making An Oral Suspension

    I'm going to go with what Burton said, but add that solutions chemistry is actually quite an esoteric field and is not as simple and wieldy as many might think it out to be. Where I'm going with this is in the future, if you're trying to prepare a solution and you're uncertain about a compound's solubility, it's best to just look up a solubility table and work with exactly the same solvent as listed in the literature. I think you're lucky your compound is soluble in ethanol. It's generally a very good thing to have a compound completely soluble in water or ethanol, and it's for human consumption in a non-research setting, because of how ethanol works in water (it's miscible). The percent might not make a big deal here. But if you were working with a compound that is insoluble in water or ethanol but soluble in an organic solvent (like oils, or ethers, or DMF), you could very easily be facing a problem if you went off book.
  9. https://doi.org/10.3389/fphar.2016.00359 Δ9-THC Intoxication by Cannabidiol-Enriched Cannabis Extract in Two Children with RefractoryEpilepsy: Full Remission after Switching to Purified Cannabidiol. Crippa JA1, Crippa AC2, Hallak JE1, Martín-Santos R3, Zuardi AW1. Author information Abstract Animal studies and preliminary clinical trials have shown that cannabidiol (CBD)-enriched extracts may have beneficial effects for childrenwith treatment-resistant epilepsy. However, these compounds are not yet registered as medicines by regulatory agencies. We describe the cases of two children with treatment-resistant epilepsy (Case A with left frontal dysplasia and Case B with Dravet Syndrome) with initial symptom improvement after the introduction of CBD extracts followed by seizure worsening after a short time. The children presented typical signs of intoxication by Δ9-THC (inappropriate laughter, ataxia, reduced attention, and eye redness) after using a CBD-enriched extract. The extract was replaced by the same dose of purified CBD with no Δ9-THC in both cases, which led to improvement in intoxication signs and seizure remission. These cases support pre-clinical and preliminary clinical evidence suggesting that CBD may be effective for some patients with epilepsy. Moreover, the cases highlight the need for randomized clinical trials using high-quality and reliable substances to ascertain the safety and efficacy of cannabinoids as medicines. So, it appears in this study that the trace THC present in CBD-enriched extract was enough to offset the beneficial effects of CBD on treatment-resistant epilepsy in these two children. By swapping this enriched extract to a purified extract, the researchers were able to restore the potent effects of CBD on controlling these two children's condition. What's not clear, and this may be detailed in the paper itself that I have yet to read, is what is this "enriched extract" and what is the purified CBD. I ask this because, at least with the THC cannabinoids, it is known that the terpenes in flowers also have their own effects that change the way you experience the cannabinoids you consume. If the extract from either the "enriched extract" or the purified CBD was processed in such a way that it did not remove the terpenes then this could also be a confounding factor. Nonetheless, it's interesting that the THC present in the enriched extracts was both enough to get them high and enough to offset the relevant medicinal effects of CBD.
  10. Growth Factor

    GF's "CS Goes Pubic" Log

    It surprisingly doesn't seem to make a difference. I did my squat session at 1030am and my last upper body at 330pm, and both times I still only slept about 4 hours. What happens is I go to bed just fine but wake up at some ungodly hour, like 3-4am, as opposed to 6 or 630am.
  11. Growth Factor

    So. Not. Powerlifting...

    Sam finally coming around to accepting his nature as a brokeback. On a serious note, sorry to hear that flaring up again. I hope the massage gives you some relief.
  12. Growth Factor

    GF's "CS Goes Pubic" Log

    18/12/10 A2 FLAT BENCH 225x8+1,2+1,1=11 rest paused reps, 2 assisted, plus static stretch, ~5 sec negs **DC chest stretch** OHP 135x7,2,1=10 rest paused reps, plus static stretch, ~5 sec negs **DC shoulder stretch** CGBP 165x7,2,2=11 rest paused reps, plus static stretch, ~5 sec negs **DC tricep stretch** NAUTILUS LAT PULLDOWN 270x9,4,3=16 rest paused reps, plus static stretch, ~5 sec negs DEADLIFT 225x5 315x3 365x1 <--bad everything **DC lat stretch** Again, aborted during the deadlifts because I started cramping everywhere and had no energy in the tank. I was pretty pleased with the weights I used on everything today. I would have wanted to start lighter on the OHPs, but I guessed my weights right on everything else. Like I said, I need to work on my nutrition and recovery to maintain this routine because it's really rough this time around. One thing that also sucks is I'm only getting about 4 hours of sleep on workout days, even if I medicate, because of how overstimulated I feel.
  13. Growth Factor

    GF's "CS Goes Pubic" Log

    18/12/07 B1 BARBELL CURLS 85x?? DB PINWHEEL CURLS 50x?? **DC bicep stretch** DC CALF PRESS ??x15, 8 second pauses in the hole LEG CURL ?? **DC hamstring stretch** HACK SQUAT ??x5 ??x18 lmao bad **DC quad stretch** Again, lost my notes and didn't record this immediately online, so I forget what I used or sometimes how much I did. One thing I remember was how bad the hack squats were, particularly on my quads and abs, so I stopped a bit early. Felt a little quad tendinitis creeping up, too.